When Maggie kills the Simpson family's enemies, she has an eye painting similar to Alex from the movie A Clockwork Orange.
Frank Grimes references to the 600th episode mark saying "In hell they make you watch them all in a row". This is possibly a reference to a 13 day marathon that happened on FXX to celebrate the milestone, where all 599 episodes of The Simpsons were aired in a row.
The name of the segment is a reference to the movie series Die Hard.
Mr. Burns reenacts one of the dance scenes from the movie Flashdance.
Lisa says that after the river dried a drought of Chicago Cubs sized proportions hit Springfield, referencing the drought of World series wins for the Cubs, although they did win the World Series in 2016
"BFF R.I P." is two acronyms for "Best Friends Forever" and "Rest In Peace".
The segment itself is probably a parody of the movie Lights Out.
The segment itself parodies action movies such as Kingsman and the James Bond series itself. With the final fight referencing the infamous church fight in Kingsman.
Homer held a Steely Dan concert at the Duff Stadium. He asks them to play Babylon Sister from the album Gaucho during the fight scene.
The promotional material for this episode shows Russ Cargill among the villans trying to kill the Simpson family, but in the final episode, he's not there.
This is the second Treehouse of Horror episode in a row where Sideshow Bob is decapitated.
The episode's script reveal that Kang was the one who appeared in the intro.
Also, this episode broke the tradition of Kang and Kodos appearing in every Treehouse of Horror episode, as only Kang appears.
Bart and Maggie do not appear during this segment.
So far, this is one of the shortest title for a Treehouse of Horror segment, with six letters, The same as Homer³ from Treehouse of Horror VI.
In Moe's lair, when Moe is kissing a picture of Homer, a picture of Dr. Marvin Monroe can be seen.
Marge, Lisa and Maggie do not appear during this segment.
Dan Castellaneta's Halloween nickname (for both his consulting producer and starring credits) is "Heeeere's DANNY!", a reference to The Shining.
Dan Vebber's nickname is "H.P Lovebbercraft", a reference to H.P Lovecraft.
Drew Carey's Halloween nickname is "Boo Scary from The Fright is Right!" which refers to his status as the host for The Price is Right.
The order of cars going to Mr. Burns's reservoir changes between scenes.
Also, the kid on top of Apu's vehicle (either Sashi or Pria) is yellow with blonde hair in one of the shots.
Wendell was not near Nelson when he helps surround Lisa, but his body is shown on Nelson's sword.
There was no reason why Lisa was blamed for the murder of Sherri and Terri. It happened right besides Janey's funeral and people probably saw that Lisa did not push the tombstone as she was talking with them.
When Lisa mounts on the motorbike, her right arm goes in the front of the bike's headlights.
The shelf at Moe's tavern was full of bottles, but on the next scene, it is full of empty beer mugs.