- Moe's run for office is an homage to the plot of the film Milk.
- A few references to "I Now Pronounce You Chuck & Larry" appears in the episode:
- The movie's plot revolves around Chuck and Larry posing as a gay couple for financial reasons, similar to Moe's adopting a gay image to enhance his business.
- Like Smithers demanding that Moe kiss him in public to prove that he's gay, Chuck and Larry are asked to kiss in public (at a city council hearing) to prove that their relationship is real. Chuck and Larry's kiss is stopped short, however, when their boss arrives, "outs" them as being straight and exposes their scheme.
- Mr. Burns signs his will as Charles Montgomery Plantagenet Schicklgruber Burns, which implies that he is related to Adolf Hitler and a descendant of the House of Plantagenet.
- Moe's line "Everybody vogue!" is a reference to the Madonna song "Vogue". The song is also heard playing in the background.
- The name of Moe's dog, Neil Patrick Hairless, is a pun on the actor Neil Patrick Harris' name. Neil Patrick Harris is also openly gay.
- After his half hour of mental competence elapses, Mr. Burns imagines himself as part of the 1914 prototypical cartoon "Gertie the Dinosaur".
- The song heard when Skinner imagines himself frolicking with Ms. Juniper is "Jennifer Juniper" by Donovan. The song is also played during the closing credits.
- At school, Bart is playing with a Buzz Spaceyear toy, a parody of Buzz Lightyear - a character from the Toy Story film series by Pixar.
- When the day and late shifts of the Springfield Nuclear Power Plant fight, the song playing is " Sing, Sing, Sing" by Benny Goodman.
- The picture of "Tom of Shelbyville" which Moe hangs up is a reference to the gay comic artist Tom of Finland.
- At the school, Skinner walks around singing a modified version of "Walking on Sunshine", an 80s pop tune by Katrina and the Waves.
- One of the gay men initially rejected at The League of Extra-Horny Gentlemen is dressed in drag as Wonder Woman.
- The picture hiding the safe where Smithers gets Burns' last will from is A Bar at the Folies-Bergère by French painter Édouard Manet, only with Burns' head replacing the woman's one.
- The part where Juniper is sitting on a mountain with her students and a guitar is a spoof of the part of the song Do-Re-Mi from the movie The Sound Of Music where Maria does the same thing.
- The title is similar to the third season episode "Flaming Moe's" and a reference to "flamer" being a somewhat derogatory slang term for gay men.
- The scene where Ms. Juniper is sitting with a guitar and kids surrounding her is a parody of The Sound of Music.
- Maggie does not appear in this episode. This is mainly because the episode focuses on Moe and Smithers instead of the Simpsons.
- Mr. Burns' full name is stated to be "Charles Montgomery Plantagenet Schicklgruber Burns". Schicklgruber is the surname of Adolf Hitler's paternal grandmother Maria Anna Schicklgruber
- Grady seems to have gained weight since the episode "Three Gays of the Condo".
- Homer, Lenny, and Carl and their late-shift counterparts (whom they fight) bear strong resemblances to each other, with only a few differences: Carl's counterpart wears glasses, Homer's has hair on the back of his head, and Lenny's has a different hair color and style.
- Moe shows pictures of his bar after previous makeovers:
- The Nag and Weasel ("Mommie Beerest")
- M ("Homer the Moe")
- Uncle Moe's Family Feedbag ("Bart Sells His Soul")
- Whiskey A Moe-Moe (A makeover Moe never actually did, but a dream he painted)
- Only two of those were failed, but The Nag and Weasel was a huge success has seen in the episode "Mommie Beerest".
- Moe previously had the idea to give the bar a gay makeover in "Days of Wine and D'oh'ses". When Barney announced he was quitting drinking, Moe said, "To stay afloat, this bar's gonna have to go queer."
- Several one-time gay characters from previous episodes reappear, including:
- Patty is a frequent guest of Mo's. At one point, Patty and Marge are mistaken for men in drag while in the bar.
- Comic Book Guy's cousin, Comic Book Gay, makes an appearance in this episode.
- Dewey Largo, who has previously been hinted as being gay and closeted, reveals his sexuality in this episode as he runs off with his partner.
- Smithers says "Hello, Moe." in Moe's speech in a similar way Sideshow Bob says "Hello, Bart." when they encounter.
- Ms. Juniper drives a Nissan Cube in the scene where she, Melody, and Skinner leave Springfield. The Cube in the episode has some styling elements from both the second generation (Z11) Cube (which was only sold in Japan) and the third-generation (Z12) Cube (which was sold in North America, Europe, and Japan). It lacks the wrap-around rear window on the passenger side that is found on the real-life Cube beginning with the second-generation model.
- When Grizzly Shaun talks to Mr. Burns in Moe's Tavern, Smithers has his normal suit on, however about two seconds later, he is wearing the suit he was when he entered the bar.