While the episode is based around only having 24 minutes the actual episode is about 21 minutes without commercial. Airing with commercials could possibly bring it up to 24.
However, the commentator says that the episode takes place between 2:34 and 3:04, 30 minutes.
Ingredients of the stink bomb: Roadkill, Burnt hair, the retainer case of Jimbo's sister, six weeks of bottled farts, possum placenta, sweat from a walk-around Goofy suit, and TCBY (That Container of Botulized Yogurt).
The upside-down newspaper Milhouse is holding is the Springfield Shopper. The headlines read "Mayor's Breath Still Problem", "Corn Less Popular" and "Bake Sale Today".
In the opening sequence, one of the CTU's computer screens shows Willie making out with Ms. Hoover.
Among the professional staff at Springfield Elementary is a secretary named Mrs. Lord.
Martin's Ant Farm brand is from Uncle Milton. In Bart on the Road, Bart calls him 'Milton' by mistake.
The name of Jimbo's mom is Carol.
This episode won the 2008 Annie Award for Best Writing in an Animated Television Production.
The entire episode is a parody of the show 24. Also can be a reference to the film 44 minutes based on the crime-themed plot as the story progresses.24, using the same episode structure and style. Jack Bauer, the main character of the show 24 makes a cameo in the episode.
Marge explaining about someone having 'tummy' trouble from eating Bart's shorts is a inside joke about Bart's common phase "Eat My Shorts!"
Somehow, the lettering on Martin's sash can be read properly in the mirror's reflection.
In one of ventilation pipes there was Miss Hoover, most likely unconscious, with Santa's Little Helper. However, she appeared in the Bake Sale. The same happens to Üter - he is in spider's web in the ventilation pipe, but is seen in the Bake Sale. Both of these could be paper, as they follow the camera.